Production of Extracellular β-mannanase by Bacillus amyloliquefaciens on a Coconut Waste Substrate
نویسندگان
چکیده
منابع مشابه
Release of extracellular enzymes from Bacillus amyloliquefaciens.
Washed-cell suspensions of Bacillus amyloliquefaciens secrete significant amounts of the extracellular enzymes alpha-amylase and protease for about 15 min in the almost complete absence of protein synthesis. This apparently represents release of preformed enzyme en route to secretion. The release was independent of energy but was affected by temperature. Pulse-labeling experiments showed that n...
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BACKGROUND Mannanase is an enzyme that can catalyze random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans which are the key polymers in hemicellulose. It has been used in a number of different industrial applications including food, feed, pharmaceutical, pulp/paper industries, and second generation biofuel. To optimize the expression sys...
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The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...
متن کاملComplementation of peptides of barnase, extracellular ribonuclease of Bacillus amyloliquefaciens.
Recovery of ribonuclease activity by complementation of peptides of barnase is reported. Activity is restored to barnase-(1-102), which lacks eight amino acids from its COOH terminus, by combination with peptides-(88-110), -(95-110), or -(99-108), and also with succinylated peptide-(88-110). The dissociation constants are about 8 X 10(-6) M for the first two combinations and little, if any, gre...
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ژورنال
عنوان ژورنال: Pakistan Journal of Nutrition
سال: 2017
ISSN: 1680-5194
DOI: 10.3923/pjn.2017.700.707